PrimerBench
PCR primer pairs ranked by nearest-neighbor Tm, with hairpin and dimer checks.
SeqBench: DNA sequence analysis in your browser
Paste a DNA sequence or FASTA record and instantly see its composition, reverse complement, translations in all six reading frames, open reading frames, and motif matches.
Free, no sign-up, and installable on your phone. Runs in your browser at seqbench.gataca.com.
What you can do with SeqBench
- Check GC content and length before ordering a synthetic gene
- Get a reverse complement or RNA transcript without retyping
- Find open reading frames in a cloned insert or contig
- Locate restriction sites, promoters, or other motifs, including IUPAC patterns
How to use it
- Paste a DNA sequence or FASTA record. Spaces, numbers, and headers are cleaned up automatically.
- Read the summary: length, GC and AT content, estimated Tm, and molecular weight.
- Switch tabs for the reverse complement, RNA, six-frame translation, or ORF list.
- Type a motif to highlight every match on both strands.
Features
- GC and AT content with a base composition bar
- Reverse complement and RNA transcription with one-click copy
- Six-frame translation using the standard genetic code
- ORF finder with adjustable minimum length
- IUPAC motif search on both strands
- Estimated melting temperature and molecular weight
- Save results as reports, spreadsheets, or sequence files
- Install it on your phone or computer; works offline
Frequently asked questions
Is SeqBench free?
Yes. SeqBench is free to use with no account or sign-up.
How is the melting temperature estimated?
Short sequences (under 14 bases) use the Wallace rule; longer ones use a GC-based formula. For primer Tm with salt correction, use PrimerBench.
How long can my sequence be?
Analysis works on sequences of any practical length; the colored sequence view shows the first 20,000 bases to keep the page fast.
More free tools
Need SeqBench to do more?
We build custom versions and full pipelines for research teams.