Paste a protein or a coding DNA sequence and get a version optimized for your expression host. The protein stays identical; only the codons change.
"Match host usage" picks codons in proportion to how often the host uses them, skipping rare codons (under 10%). "Most frequent codon" always uses the host's top codon, which maximizes CAI but can create repetitive sequence. Change the seed to get a different valid design.
Highlighted codons are used less than 10% of the time for their amino acid in the selected host.
Codon usage values are approximate relative frequencies based on the Kazusa codon usage database. Check critical constructs against your synthesis vendor's tools.
CodonBench is a free online tool for codon optimization. Optimize a gene's codons for E. coli, yeast, or human expression. It runs entirely in your browser, so there is nothing to install and your data stays on your device.
Most frequent codon always uses the host's top codon, which maximizes CAI but can create repetitive sequence. Match host usage chooses codons in proportion to natural usage and skips rare ones, which usually gives a more balanced gene.
No. Every design is translated back and checked against your original protein before it is shown.
They are approximate relative frequencies based on the Kazusa codon usage database. For critical constructs, confirm with your gene synthesis provider.